Mouse FITC Monoclonal Antibody
Species Reactivity Chemical
Published species Chemical
Host/Isotype Mouse / IgG2a, kappa
Recommended Isotype Control Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™
Conjugate PESee Additional Formats
Excitation/Emission ProfileView spectra
Concentration 0.2 mg/mL
Purification Affinity chromatography
Storage buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage conditions 4° C, store in dark, DO NOT FREEZE!
Product Specific Information
Description: This NAWESLEE monoclonal antibody reacts to fluorescein isothyocianate (FITC), a derivative of fluorescein commonly used in flow cytometry and fluorescent microscopy. FITC-9 can be used for the separation of cells labeled with FITC-conjugated antibodies or for staining. Please note that in some instances the FITC signal can be quenched by the anti-FITC antibody; therefore, this phenomenon should be evaluated empirically.
Applications Reported: This NAWESLEE antibody has been reported for use in flow cytometric analysis.
Applications Tested: This NAWESLEE antibody has been tested by flow cytometric analysis of cells stained with FITC-conjugated antibody. This can be used at less than or equal to 0.5 µg per test. Our testing indicates that, if used at higher concentration, binding of the NAWESLEE monoclonal antibody can quench FITC fluorescence. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.